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KMID : 0878220100220040077
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Journal of Sasang Constitutional Medicine
2010 Volume.22 No. 4 p.77 ~ p.84
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Stimulation of the Immune Response by Yanggyuksanhwa-tang
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Jung Da-Young
Ha Hye-Kyung
Lee Ho-Young
Lee Jin-Ah
Lee Nam-Hun
Lee Jun-Kyoung
Huang Dae-Sun
Shin Hyeun-Kyoo
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Abstract
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Objectives: Yanggyuksanhwa-tang for Soyangin was applied to investigate the immunological activities on antigen (Ag)-specific or Ag-non-specific immune responses on murine macrophage cell line (RAW 264.7) and ovalbumin/aluminium (OVA/Alum)-immunized mice.
Methods: This study were carried out in nitric oxide (NO) synthesis on RAW 264.7 cells and cellular proliferation on mouse splenocytes. C57BL/6 mice were immunized intraperitonially with OVA/Alum (100 /200 ) on day 1, 8, and 15. Yanggyuksanhwa-tang was administrated to mice orally for 3 weeks from day 1. On day 22, OVA-, lipopolysaccharide (LPS)-, and concanavalin A (Con A)-stimulated splenocyte proliferation and antibodies (OVA-specific antibodies of the IgG, IgG1, and total IgM classes) in plasma were measured.
Results: Yanggyuksanhwa-tang significantly enhanced cellular proliferation by LPS and Con A on splenocytes from OVA/Alum-immunized mice (p<.001). Yanggyuksanhwa-tang also significantly enhanced plasma OVA-specific IgG (p<.001), IgG1 (p<.001), and total IgM (p<.01) levels compared with the OVA/Alum group.
Conclusions: These results suggested that Yanggyuksanhwa-tang for Soyangin could be used as immunopotent.
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KEYWORD
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Yanggyuksanhwa-tang (Lianggesanhuo-tang), Antibodies, LPS, Con A, OVA
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